It is easy to find from Eqs. (5) and(6) that the absolute molar
concentrations of various species are strongly required to calculate
the binding constants.Fortunately,earlier work lets us know that
the information about stoichiometric numbers of all species in
the reaction and the known absolute concentration of AuNPsas
reference allow us to deduce the absolute concentration evolutions and the spectra in absolute unit of all species,resulting in the
elimination of intensity ambiguity