Since the storage temperature (25 °C) is significantly lower than
the crystallization temperature (32 °C) it is expected that further crystallization
occurs during the tempering process (secondary crystallization
Even though secondary crystallization occurs in these samples,
the effect of sonication is maintained during this process and the difference
in crystal sizes is maintained (Fig. 1). However, when samples are tempered at 5 °C, the degree of secondary crystallization is increased
and therefore the effect of sonication is partially lost and even though
harder or more elastic lipid networks are obtained due to the lower storage
temperature, the effect of sonication on the viscoelastic properties
of the material is lost. In short, US can be used in combination with
and different storage temperatures to obtain various degrees of
viscoelasticity