For the samples of industrially processed cocoa, the technique of
dilution plating was applied. Under aseptic conditions, 25 g of each
sample were weighed and 225 mL of sterile peptone water 0.1%
were added. Then, aliquots of the serial dilutions were prepared
and inoculated on plates containing DG18. The plates were incubated
at 25 C for 7 days and the results expressed in colony
forming units per gram of sample (CFU/g), according to