As the fleshy sarcotesta develops, a considerable accumulation
of pigments takes place, so that when the seed is ready for
dispersal the sarcotesta is bright red. Pigments were extracted
with either a solvent (diethyl ether) for carotenoids or acid
methanol for anthocyanins, and separated by chromatography
with known samples for comparison. Diethyl ether extracted the
red pigment of the sarcotesta, which was analyzed by
chromatography. The control sample consisted of pigments
extracted from young leaves, and carotenoids appeared to be
located in the front of the run, like those extracted from the red
sarcotesta. Chlorophylls only appeared in the lane with foliar
pigments (Fig. 4D).
As the fleshy sarcotesta develops, a considerable accumulationof pigments takes place, so that when the seed is ready fordispersal the sarcotesta is bright red. Pigments were extractedwith either a solvent (diethyl ether) for carotenoids or acidmethanol for anthocyanins, and separated by chromatographywith known samples for comparison. Diethyl ether extracted thered pigment of the sarcotesta, which was analyzed bychromatography. The control sample consisted of pigmentsextracted from young leaves, and carotenoids appeared to belocated in the front of the run, like those extracted from the redsarcotesta. Chlorophylls only appeared in the lane with foliarpigments (Fig. 4D).
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