4. Discussion
Micropropagation in tea dates back to the early 80s with
numerous advancements to date [11]. However, with the
development of new clones through breeding programs,
response to micropropagation can differ. This paper reports
on a rapid and efficient method for micropropagation of tea
clone Iran 100 which incorporates the stage of multiplication
and elongation into a single step protocol to reduce the cost
of production. The two most commonly used cytokinins,
namely, BAP and TDZ, reported to have positive effect on
multiplication on different tea clones were tested.
Multiplication of primary explants is highly dependent on
plant species and the type of cytokinins used. Several reports
have been published on in vitro culture of tea and related