purified in MRS broth (Acumedia). To identify LAB within the
isolated strains, preliminary tests including microscopic morphology
characteristics, Gram staining, catalase production, CO2
production from glucose and organic acid (lactic and acetic)
production were performed. Only seven wild coconut LAB
strains were detected and subsequently tested for viability after
a freeze challenge. These strains were cultured onto MRS broth
and stored for 48 h at −80 °C.