Identification of the bacterial groups is done through observation of colony morphology, the colony color,
colony diameter and the diameter of the resulting slime and form colonies, gram test with KOH, to determine that
the gram-negative bacteria produce exopolysaccharide Gram test using 3% KOH done by: Combine 1 loop pure
cultures of bacteria with 1 drop of 3% KOH (w / v in H2O), and then stirred repeatedly using a needle ose. Lift
loopful many times from the suspension surface, observe whether the bacteria form a sticky suspension lifted like a
thread with a needle ose. When the suspension turned been like a slimy, sticky and lifted like a thread with a needle
ose, meaning Gram negative (-). Conversely, if the suspension remains dilute, not rose like the needle thread loop,
meaning Gram positive (+). The selected bacterium is gram-negative bacteria. To prove and validate whether these
bacteria are gram-negative bacteria producing exopolysaccharide subsequently grown on the Mac Concey medium
(Oxoid CM 0007) is characterized by the presence of slime formed
Identification of the bacterial groups is done through observation of colony morphology, the colony color,colony diameter and the diameter of the resulting slime and form colonies, gram test with KOH, to determine thatthe gram-negative bacteria produce exopolysaccharide Gram test using 3% KOH done by: Combine 1 loop purecultures of bacteria with 1 drop of 3% KOH (w / v in H2O), and then stirred repeatedly using a needle ose. Liftloopful many times from the suspension surface, observe whether the bacteria form a sticky suspension lifted like athread with a needle ose. When the suspension turned been like a slimy, sticky and lifted like a thread with a needleose, meaning Gram negative (-). Conversely, if the suspension remains dilute, not rose like the needle thread loop,meaning Gram positive (+). The selected bacterium is gram-negative bacteria. To prove and validate whether thesebacteria are gram-negative bacteria producing exopolysaccharide subsequently grown on the Mac Concey medium(Oxoid CM 0007) is characterized by the presence of slime formed
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