Moreover, cytokine gene expression from the BAL fluid was performed. Total antibodies
against M. hyopneumoniae and specific IgG were detected in serum of intradermally and
intramuscularly (vaccine B only) vaccinated pigs at 4 and 8 weeks post-vaccination. M.
hyopneumoniae specific IgA were detected in BAL fluid from vaccinated animals (Groups A
and B) but not from controls and animals vaccinated with the bacterin D (p < 0.05)