Oil extraction was conducted using a Soxhlet
apparatus for about 5 h with hexane as solvent at a
solid to solvent ratio of 1/7 m/v. After the extraction
process, the flask contents were filtered, and
the liquid fraction containing the lipid extract and
solvent was poured into a 250-mL flask of a rotary
film evaporator to remove the solvent. The obtained
oil was collected, evaporated under nitrogen,
weighed, and stored in sealed amber glass vials at
-20°C until analysis.