For eGFP library expression,freshly transformed DH10B colonies
were inoculated intriplicate into 5mL LB media and weregrown at
37 1C for 24h.125mL of each culture was aliquotted into 96-well
plates and measured on a BiotekSynergy H4 plate reader for OD600
and fluorescence at 488 nm excitation and 509 nm emission.