Human breast cancer cell lines MCF-7 and MDA-MB-231 were
procured from National Centre for Cell Sciences (NCCS Pune, India)
and were cultured in Dulbecco's Minimum Essential Medium
(DMEM) supplemented with 10% Fetal Bovine Serum (FBS), Penicillin
(100 U/ml) and Streptomycin (100 mg/ml). The cells were
maintained at 37 C in a humidified incubator with 5% CO2 and. 80%
confluent cells were sub cultured by trypsinization.