For most blocks, only one section was needed; for
a limited number of blocks two or three sections were
needed (>1000 cells: one section, 500e1000 cells: two
sections, 300e500 cells: three sections).
Sections of 6 mm in thickness were cut from FFPE
blocks using the microtome and mounted on UV-treated
Polyethyl naphthalate (PEN)-membrane slides. Paraffin
was removed by incubating slides at 56 C for 3e6 h
before staining according to standard H&E protocol.
Sections were air-dried and submitted for LCM on the
same day.