The method was to catch prawn quickly and haemolymph at once were sampled from abdominal cavity or around heart
ventricle by 1 mL syringe adopting 4 C Alsever anticoagulant (27 mM Na citrate, 336 mM NaCl,115 mM glucose, 9 mM EDTA, pH 7) with the proportion of haemolymph to anticoagulant at 1:1 [21]