Fermentation media formulation from some organic nutrient sources in partial
or full replacement of nitrogen requirements is receiving attention as a means of
backward integration. Submerged Culture fermentation was used to study the
effect of nitrogen replacement of amylase fermentation medium using organic
sources. Aspergillus niger isolated from the soil was used as the chemical
factory while rice bran was used as the carbon source. The amylase activity of
the nitrogen sources were low although. Cotton seed gave an amylase activity
of 1.282 IU/ml which was the highest recorded for this work. Peptone had
amylase activity of 0.962 IU/ml whereas Blood and Ground-nut cake (Kuli-
Kuli) had an amylase activity of 0.713IU/ml and 0.512IU/ml respectively. Rice
bran for this work appears to be a good carbon source and a good replacement
for starch as the quality of enzyme produced compared well with starch
substrate. Amylase production level was 33.1% higher in activity when peptone
was replaced with cotton seed. Cotton seed may be used in full replacement of
Peptone.
Cite This Article as: Damisa D, HB Sharif and RH