BHI medium was prepared by dissolving 74 grams of BHI Agar (BBLTM Cat. No. 211065) in two liters of distilled water followed by autoclaving at 121 °C for 15 minutes. Potato dextrose agar with chloramphenicol was prepared by dissolving 78 grams of Potato Dextrose Agar (DifcoTM Cat No. 213400) in 1.99 liters of distilled water. One gram of chloramphenicol (Sigma Chemical Co, St. Louis, MO) was dissolved in 10 ml of distilled water and added to the potato agar solution prior to autoclaving at 121 °C. Both media were poured into sterile plastic Petri dishes and allowed to harden, and then tested for sterility before use.