The lacZ gene fragment was prepared by digesting
the plasmid pUC19 with the restriction enzymes, NdeI/HindIII
which covered the entire section of the lacZ gene in pUC19, to obtain
a size of 264 base pairs. The fragment was detected and confirmed by
gel electrophoresis in a comparison with the DNA marker (Lambda/
PstI). The lacZ gene fragment was eluted from gel and purified using
the Silica Bead DNA Gel Extraction Kit (Fermentas, Glen Burnie,
Maryland, USA) and ready for ion bombardment.