350 rpm in modified 250-mL baffled Erlenmeyer flasks. The
modified Erlenmeyer flasks were fitted with oxygen sensors
(PSt3, PreSens, Regensburg, Germany) that provided continuousmonitoring
of dissolved oxygen (DO) tension in shake flask
cultures by transferring DO data via Bluetooth at 15-min intervals
to the SFR v2 system (PreSens), as previously described
(Jackson 2012). The oxygen concentration CO2; L was measured
as O2 molecules in the liquid phase, which was expressed
as percent dissolved oxygen. The time of fermentation required
to reach 50 % DO (DO50) was computed. All B. bassiana cultures
were incubated at 28 °C in a rotary shaker incubator.