have been considered for the identification of certain bacterial taxons. Such genes may provide well conserved regions with potential usefulness for the design of primers and molecular probes for identification purposes in Bacillus spp. Accordingly, the main objective of this work was to evaluate the usefulness of different DNA regions, mainly the tuf, rpoB and gyrB genes, to achieve a good discrimination of foodborne Bacillus spp. with relevance for food safety.