Fresh leaves were processed with a Clevenger apparatus, and the EO was obtained by hydrodistillation, protected against light and heat, and stored until behavioral assays. Previously, the EO was analyzed by gas chromatography coupled with mass spectrometry (GC/MS) as described (Blanco et al., 2009).
Thin layer chromatography (TLC) of EO and decoction was performed on Silica gel 60 (250 m, 10 cm × 20 cm, Sigma–Aldrich®