Intestinal mucous cell numbers and their glycoconjugate composition were investigated by histochemical
methods in uninfected chub, Squalius cephalus, and in conspecifics naturally parasitised with the acanthocephalan
Pomphorhynchus laevis. A sub-population of 42 chub from the River Tiber (Perugia, Italy) were sampled and
screened for ecto and endoparasites. No parasites were found in gills and in other visceral organs of chub and
P. laevis appeared to be the only enteric worm encountered. In all infected chub (twenty-eight out of 42) this
acanthocephalan was encountered mainly in the mid-gut. In situ, an excessive yellowish mucus or catarrh was
observed around each acanthocephalan. Hyperplasia and hypertrophy of the mucous cells were only evident
near the site of P. laevis attachment where the total number of mucous cells and the number of those containing
acidic, particularly non-sulphated mucins, or mixed glycoconjugates were significantly higher. In intestinal
regions of infected fish far away from the point of parasite attachment, there were no statistical differences in
the density of mucous cells in comparison to uninfected fish. Interestingly, in parasitised chub, the length of
intestinal folds was significantly larger close to the sites at which P. laevis attach when compared to the length
of the intestinal folds located further away from the acanthocephalans and/or in uninfected intestines. The effect
of P. laevis on intestinal mucous cells of S. cephaluswas compared to other parasite–host systems and the role of
enhanced mucus production in parasitized intestines was discussed
Intestinal mucous cell numbers and their glycoconjugate composition were investigated by histochemicalmethods in uninfected chub, Squalius cephalus, and in conspecifics naturally parasitised with the acanthocephalanPomphorhynchus laevis. A sub-population of 42 chub from the River Tiber (Perugia, Italy) were sampled andscreened for ecto and endoparasites. No parasites were found in gills and in other visceral organs of chub andP. laevis appeared to be the only enteric worm encountered. In all infected chub (twenty-eight out of 42) thisacanthocephalan was encountered mainly in the mid-gut. In situ, an excessive yellowish mucus or catarrh wasobserved around each acanthocephalan. Hyperplasia and hypertrophy of the mucous cells were only evidentnear the site of P. laevis attachment where the total number of mucous cells and the number of those containingacidic, particularly non-sulphated mucins, or mixed glycoconjugates were significantly higher. In intestinalregions of infected fish far away from the point of parasite attachment, there were no statistical differences inthe density of mucous cells in comparison to uninfected fish. Interestingly, in parasitised chub, the length ofintestinal folds was significantly larger close to the sites at which P. laevis attach when compared to the lengthof the intestinal folds located further away from the acanthocephalans and/or in uninfected intestines. The effectof P. laevis on intestinal mucous cells of S. cephaluswas compared to other parasite–host systems and the role ofenhanced mucus production in parasitized intestines was discussed
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