In attempts to reproduce Negri bodies in embryonic nervous tissue cultures of mice, the authors used 15-18-day-old embryos and fowl plasma was found to be the most favourable medium. Tissue cultures were infected with street virus and Negriform bodies were observed after the sixth day. Similar bodies were also observed in the control tissue cultures. Further study showed that they never appeared in cultures of chick embryo nervous tissue. They were observed only in cultures where neuro-epithelial tissue formation was well established. They were equally found in cultures from which all extraneous elements had been removed. The presence of these bodies could not be demonstrated in cultures of mouse nervous tissue in a base of rabbit or mouse plasma. It was concluded that the bodies observed were the result of tissue culture on a heterologous medium.-G. V. LAUGIER.
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