During a 2-wk depletion period, all pigs (initial BW:
31.1 ± 4.2 kg) were housed individually in 0.9 by 1.8
m pens that had fully slatted concrete floors, a stainless
steel feeder, and a stainless steel bowl-type drinker. All
pigs were fed the corn grits diet containing BMM during
this period. Pigs were then transferred to metabolism
cages and randomly allotted to the 6 experimental diets.
The average weight of the pigs at the time of transfer was
40.2 ± 6.2 kg. The stainless steel metabolism cages were
equipped with a fully perforated floor, a screen floor for
fecal collection, and a stainless steel tray for urine collection.
The quantity of feed provided per pig daily was
calculated as 3 times the estimated requirement for maintenance
energy (i.e., 106 kcal ME per kg0.75; NRC, 1998)
and divided into 2 equal meals. Water was provided by a
bowl-type drinker and available at all times. Experimental
diets were provided for 12 d. The initial 5 d was considered
an adaptation period to the diet. Fecal markers were
fed on d 6 and on d 11 and fecal collections were initiated
when the first marker appeared in the feces and ceased
when the second marker appeared (Adeola, 2001). Urine
was collected from d 6 to 11 in urine buckets over a preservative
of 50 mL of 6 N HCl. Buckets were covered
by gauze to prevent solids from contaminating the urine.
Fecal samples and 20% of the collected urine were stored
at –20°C immediately after collection.
During a 2-wk depletion period, all pigs (initial BW:31.1 ± 4.2 kg) were housed individually in 0.9 by 1.8m pens that had fully slatted concrete floors, a stainlesssteel feeder, and a stainless steel bowl-type drinker. Allpigs were fed the corn grits diet containing BMM duringthis period. Pigs were then transferred to metabolismcages and randomly allotted to the 6 experimental diets.The average weight of the pigs at the time of transfer was40.2 ± 6.2 kg. The stainless steel metabolism cages wereequipped with a fully perforated floor, a screen floor forfecal collection, and a stainless steel tray for urine collection.The quantity of feed provided per pig daily wascalculated as 3 times the estimated requirement for maintenanceenergy (i.e., 106 kcal ME per kg0.75; NRC, 1998)and divided into 2 equal meals. Water was provided by abowl-type drinker and available at all times. Experimentaldiets were provided for 12 d. The initial 5 d was consideredan adaptation period to the diet. Fecal markers werefed on d 6 and on d 11 and fecal collections were initiatedwhen the first marker appeared in the feces and ceasedwhen the second marker appeared (Adeola, 2001). Urinewas collected from d 6 to 11 in urine buckets over a preservativeof 50 mL of 6 N HCl. Buckets were coveredby gauze to prevent solids from contaminating the urine.Fecal samples and 20% of the collected urine were storedat –20°C immediately after collection.
การแปล กรุณารอสักครู่..
