To produce good quality and higher yield of alcohol from rice straw, delignification plays a dominant role including all processes resulting in partial or total removal of lignin from lignocellulosic biomass by the action of appropriate chemical reagents or physical means while retaining the most fermentable sugars. Among the chemical treatments, oxidative treatments are principally affecting the degradation of lignin, whereas hydrolytic agents cleave the lignin-carbohydrate linkages. Coupling of the hydrolysis and the oxidative treatment gives better delignification efficiency. In very recent year, superiority of different pretreatment types for lignocellulosic materials using alkaline solution has been made based on the enzymatic digestibility [14]. Therefore, choosing the right pathway of delignification for efficient and cost effective utilization of cellulosic biomass towards bio-fuel production is the present day challenge.