Initially, a kit containing
dual-color ERG break-apart probes that can identify
rearrangement of the ERG gene but do not indicate the
5’-partner to which ERG is fused to, was used [48,49].
Later on, a tricolor FISH assay was developed by combining the red/green break-apart probes for TMPRSS2
with an orange-labeled fusion probe for the 3’ region of
ERG [50,51]. Recently, a four-color FISH assay was
reported, which allows the detection of either TMPRSS2
or ERG rearrangements regardless of the partner gene
Initially, a kit containingdual-color ERG break-apart probes that can identifyrearrangement of the ERG gene but do not indicate the5’-partner to which ERG is fused to, was used [48,49].Later on, a tricolor FISH assay was developed by combining the red/green break-apart probes for TMPRSS2with an orange-labeled fusion probe for the 3’ region ofERG [50,51]. Recently, a four-color FISH assay wasreported, which allows the detection of either TMPRSS2or ERG rearrangements regardless of the partner gene
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