ASCs (passage 2) were stimulated for 24 h with 10 ng/ml IFNg and IL-1b and 1 mg/ml recombinant murine S100A8. Bone marrow cells were harvested from C57Bl6J mice and 1 106 were differentiated into macrophages with M-CSF for 6 days and activated with 10 ng/ml LPS for 24 h. Consequently, these macrophages were incubated for 24 h with IFNg- and IL-1b- stimulated ASC supernatant. Effect on gene expression of suppressive factors in both ASCs and macrophages was investigated using reverse transcription (RT)-qPCR.