100 μL of linoleic acid were dissolved in 4 ml of EtOH, 8 ml of 0.05 M phosphate buffer (pH 7.0) and 3.9 ml of distilled water. 50 μL of sample (10, 25, 50 mg/ml) was added to 1.4 mL of the previously described linoleic acid solution. This mixture was kept in darkness and at 40 °C; the accelerated oxidation of linoleic acid was measured after 24, 48, 72 and 96 h of thermal treatment.