A comparison of the rosette phenotypes of the pp2a-b'γ, pp2a-b'ζ1-1, pp2a-b'ζ 1-2, and the pp2a-b'γζ double mutant grown under low and high light conditions. (A) Plants grown for 2 weeks under low light (250 μmol m−2 s−1) and then either maintained for a further 7 days under low light growth conditions (top row) or transferred to high light (800 μmol m−2 s−1) for 7 days (bottom row). A pp2a-b'γζ double mutant was constructed by crossing the SALK_039172 (pp2a-b'γ) and SALK_107944C (pp2a-b'ζ 1-1) single mutants. (B) RT-PCR analysis of PP2A-B'ζ mRNA in wild type and pp2a-b'γ, pp2a-b'ζ1-1, pp2a-b'ζ1-2, and the pp2a-b'γ ζ double mutant. Amplification of Actin 2 is used to control for equal cDNA-levels. (C) RT-PCR analysis of PP2A-B'γ mRNA in wild type, pp2a-b'γ, pp2a-b'ζ1-1, and the pp2a-b'γ ζ double mutant. Band intensity values of the PP2AB'γ-amplicon of each sample were normalized to Actin 2 values. Average values ± standard error values are shown (n = 3–4). The asterisk denotes a value that is significantly lower than the wild type-value (P < 0.05) as determined by a Student's t-test.
A comparison of the rosette phenotypes of the pp2a-b'γ, pp2a-b'ζ1-1, pp2a-b'ζ 1-2, and the pp2a-b'γζ double mutant grown under low and high light conditions. (A) Plants grown for 2 weeks under low light (250 μmol m−2 s−1) and then either maintained for a further 7 days under low light growth conditions (top row) or transferred to high light (800 μmol m−2 s−1) for 7 days (bottom row). A pp2a-b'γζ double mutant was constructed by crossing the SALK_039172 (pp2a-b'γ) and SALK_107944C (pp2a-b'ζ 1-1) single mutants. (B) RT-PCR analysis of PP2A-B'ζ mRNA in wild type and pp2a-b'γ, pp2a-b'ζ1-1, pp2a-b'ζ1-2, and the pp2a-b'γ ζ double mutant. Amplification of Actin 2 is used to control for equal cDNA-levels. (C) RT-PCR analysis of PP2A-B'γ mRNA in wild type, pp2a-b'γ, pp2a-b'ζ1-1, and the pp2a-b'γ ζ double mutant. Band intensity values of the PP2AB'γ-amplicon of each sample were normalized to Actin 2 values. Average values ± standard error values are shown (n = 3–4). The asterisk denotes a value that is significantly lower than the wild type-value (P < 0.05) as determined by a Student's t-test.
การแปล กรุณารอสักครู่..
