defined as the limit at which the difference between two different values may reasonably well be identified and determined by considering five times the product value of standard deviation of analysis of seven standard samples containing the similar analyte concentration through the
entire analytical process and one-sided t-distribution (t=3.14 for six degrees of freedom), standard deviation, relative standard deviation and correlation coefficient (R), of the
method were calculated for nitrate calibration curve equation,CNO2−=8.24 Abs+0.16.