Virus culture
The viral laboratories of TCVGH and CMUH were both
qualified contract laboratories by Centers for Disease
Control of Taiwan. Throat swab specimens were stored at
4C in the refrigerator no more than 3 days before inoculation.
Four cell lines, including monkey kidney epithelial
cell (Vero), human rhabdomyosarcoma cell (RD), human
epidermoid carcinoma cell (A-549), and MadineDarby
canine kidney cell, were routinely used for virus isolation.
The cytopathic effect of cell culture was checked daily and
confirmed by direct fluorescence assays of suspect virus.