2.2.5. Chlorophyll
Chlorophyll content in chillies was measured following the
method Ten grams sample was
taken and extracted with acetone in a blender. The extraction
was repeated with acetone till the residue was colourless. The
filtered contents were taken in a separating funnel along with
50 ml of diethyl ether. Water was added 5–10 times to remove
the acetone layer until the ether layer is free of acetone. The
ether extract was transferred to a 100 ml volumetric flask and
made up the volume with ether. Anhydrous Na2SO4 was added
to remove the traces of moisture. The OD (optical density) was
measured using spectrophotometer (Shimadzu 1609, Tokyo,
Japan) at 660 and 642.5 nm. The total chlorophyll was
calculated using following formula: