Pre-treatment of polyethylene:The polyethylene bags were cut in small strips and transferred to a fresh solution containing 70 ml Tween 80, 10 ml bleach, and 983 ml distilled water and stirring for 30 to 60 minutes (El-Shafei et al., 1998). The strips were transferred to a beaker with distilled water and stirred for 1 hour. Further, they were aseptically relocated to ethanol solution 70% v/v for 30 min. Finally, the polyethylene strips were transferred to a Petri dish
and incubated at 45 to 50oC overnight.