Immunoassays, such as enzyme-linked immunosorbent assays
(ELISA), have been developed for rapid detection of L. monocytogenes.
These methods target antibodies specific to Listeria, and
although less time-consuming than classical culturing, they are
less sensitive with detection limits of 104
–106 CFUml1 [20].
Detection of L. monocytogenes in artificially contaminated milk
samples using MALDI-TOF MS was recently reported [21].
Here, two enrichment steps were required and detection of
L. monocytogenes in milk spiked with an initial inoculum of
1 CFUml1 was possible after 30 h. The detection of VOCs liberated
from enzyme substrates could potentially be used as a rapid
method for the detection of L. monocytogenes. However, currently
the method has several limitations; these include use of a small
sample size and a limited range of reference bacteria. Testing the
method with a larger sample size, in addition to examining the
VOCs generated by other strains of all Listeria species would
be necessary. In addition, samples used to test the method were
artificially contaminated; further investigations would be required
using real-life samples
Immunoassays, such as enzyme-linked immunosorbent assays(ELISA), have been developed for rapid detection of L. monocytogenes.These methods target antibodies specific to Listeria, andalthough less time-consuming than classical culturing, they areless sensitive with detection limits of 104–106 CFUml1 [20].Detection of L. monocytogenes in artificially contaminated milksamples using MALDI-TOF MS was recently reported [21].Here, two enrichment steps were required and detection ofL. monocytogenes in milk spiked with an initial inoculum of1 CFUml1 was possible after 30 h. The detection of VOCs liberatedfrom enzyme substrates could potentially be used as a rapidmethod for the detection of L. monocytogenes. However, currentlythe method has several limitations; these include use of a smallsample size and a limited range of reference bacteria. Testing themethod with a larger sample size, in addition to examining theVOCs generated by other strains of all Listeria species wouldbe necessary. In addition, samples used to test the method wereartificially contaminated; further investigations would be requiredusing real-life samples
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