2.4. Confocal fluorescence microscopy
Confocal laser scanning microscopy was performed with a confocal microscopic system (fv1000, Olympus, Tokyo, Japan). For fluorescence excitation, He/Ne laser at
561 nm was used for kisspeptin, Ar visible laser at 488 nm for NKB. Images were collected and stored on a magnetic mass memory, and processed by the Olympus confocal software.