Fig. 2. HPLC chromatogram of the extracts frompreparative SFE. Sample: methanol
solution of preparative SFE extraction without any further treatment. Column: kromasil
100-C18 column (250mm×4.6mm I.D., 5m); mobile phase: phosphate
buffer (pH 6.5):methanol = 17:3 (v/v); flowrate: 1.0mlmin−1; detectionwavelength:
260 nm.