Our present study indicated that fish oil replacement by dietary CLA could enhance fat content, shear force and cis-9, trans-11 CLA and trans-10, cis-12 CLA inclusion in musculature, thereby improving some meat quality traits of L. vannamei. Replacing fish oil by both 1% and 2% CLA can be efficacious. However, 2% CLA inclusion may have negative effects on meat quality. For example, significant enhancement of shear force could result in tough meat, and significant reduction of 20:5 n−3 could decrease the health value of shrimp meat. Therefore, 1% CLA was the most appropriate in improving some meat quality traits.