MATERIAL AND METHODS
Animal, diets and sampling procedure
Twenty Large White and final hybrids were used
in this experiment, including 10 gilts and 10 castrates.The RYR1 genotype (malignant hypertherby a DNA based test (Genetic Department, RIAP
Nitra) described previously (Bauerova et al., 1999).
To create homogeneous groups as for the frequency
of occurrence of mutation on RYR1 gene and the
sex of pigs two groups were formed with 6 normal
and 4 heterozygotes on MH and with equal
number of gilts and castrates. The pigs (average live
weight of 35 kg) were penned and fed in boxes at
breeding cooperative farm (PD Devio Nove Sady).
Pigs were fed a low vitamin E (30.9 mg/kg) and
selenium (0.18 mg/kg) basal diet (Table 1). Animals
of group Sel received the basal diet supplemented
with 0.3 mg organic Se/kg diet. Feeding (Schauer
technology) and water were ad libitum. The facilities
met the requirements of the animal care. Animals
were stunned, slaughtered and exsanguinated in
the slaughterhouse of RIAP Nitra (transportation
about 10 km) with the mean live weight of 105 kg.
Following slaughter, the carcasses were chilled at
4°C for 24 h, and then the m. longissimus lumborum
et thoracis (MLLT) and m. semimembranosus (MSM)
were removed from each carcass. A portion of theTable 1. Composition of the basic diet
Feed composition
Wheat (%) 35
Barley (%) 32
Soybean meal (%) 16
Oat (%) 8
Wheat meal (%) 4
Mineral and vitamin mix (%) 3
Meat and bone meal (%) 2
Chemical composition
Crude protein (%) 17.5
Crude fat (%) 2.3
Crude fibre (%) 4.6
Ash (%) 8.2
Vitamin E (mg/kg) 30.9
Lysine (mg/kg) 9.1
Threonine (mg/kg) 6.4
Methionine (mg/kg) 2.8
Tryptophan (mg/kg) 2.2
Selenium (mg/kg) 0.18
sample was used immediately and the remaining
sample was wrapped in aluminium foil and stored
in a refrigerator at 4°C for 5 days.