Place a tongue depressor on the extended tongue and with a sterile cotton
swab; obtain a specimen from the pharyngeal tonsil by rotating the swab
vigorously over its surface without touching the tongue
2. Inoculate a tube of sterile saline with the swab and mix until you have a
uniform suspension
3. Using a sterile inoculating loop, inoculate one plate each of blood agar,
mannitol salt agar, and Mueller-Hinton tellurite or Tinsadale agar, all
previously labeled with the source of the specimen, by means of a four-way
streak inoculation
4. Using a sterile cotton swab moistened in sterile saline, obtain a specimen from
the skin by rubbing the swab vigorously against the palm of your hand
5. Inoculate a tube of sterile saline with the swab and mix
6. Inoculate one plate each of blood agar, mannitol salt agar, and Sabouraud
agar, as described in step3
7. Incubate the inverted Sabouraud agar plate for 48 hours at 25oC and the
remaining plate for 48 hours at 37oC12. Blot dry with bibulous paper and
examine under oil immersion.