Identification and quantification of volatile
compounds in the pito samples were done according
to a protocol described by Duarte et al., (2010) with
some modifications. In order to identify the major
volatile compounds, the samples were analyzed
directly without any pretreatment. A Varian CP-3800
gas chromatograph equipped with a Split/Splitless
injector, a flame ionization detector, and a capillary
column (30 m x 0.25 mm i.d., 2.5 μm film thickness;
Chrompack) coated with CP-Wax 52 CB was used.
Nitrogen was used as the carrier gas at a constant
flow rate of 2.0 mL min-1. Injections of 1μL were
made in the splitless mode (vent time, 60 s) and the
volatile compounds were identified by comparing the
retention times of the samples with those of standard
compounds.