Amore detailed knowledge about the Bio systems is essential to understand and control degradation processes (Othmane etal.,2007 ).In conventional of ‘‘one-factor at a time’’ approach, certain medium components and cultural parameters are used by optimizing one factor at a time, and keeping other variables constant. The traditional technique is simplest to implement. However ,a multivariable system is not only time restrictive, but also often easy to ignore the interactive effects among various parameters (Mohana etal.,2008 ).Response Surface Methodology (RSM)is a useful model for studying the effect of several factors influencing the process of seeking the optimal conditions. This approach reduces the number of experiments, improves statistical interpretation possibilities, and indicates the interaction between multiple variables (Bandaru etal.,2006;Vishwanath aetal.,2010 ).