Two effective consortiums resulting from the
above dual culture studies were selected and tested
for their suppressing ability using bacterial culture
filtrate (BCF). An agar plug (9 mm diameter) taken
from actively growing fungal culture was placed on
the surface of the plate-enhancing medium.
Bacteria initially were grown on NB medium and
incubated for 24 hours. One ml of culture
suspension was centrifuged at 8944g for 5 minutes.
A total of 100 mL supernatant of BCF was
transferred into a petri dish and then poured to the
homogenized PDA medium. Plates inoculated with
fungal agar plugs alone were used as control. A
piece of P. oryzae isolate was placed by using a
cork bore diameter of 0.5 cm into the PDA medium
in petri dishes. Each treatment was performed using
8 replications. The entire petri dish containing
treatment was incubated for one week at room
temperature, and then the radial growth of P.
oryzae was measured and percent inhibition of
pathogen growth was calculated (Kumar et al.,
2000).