Gichner et al., (2000) have found that Maleic hydrazide (MH) induced a high
frequency of somatic mutations in leaves of tobacco and a high yield of chromosome
aberrations in roots of field bean. Using Methyl methanesulfonate (MMS), ethyl
methanesulfonate (EMS), N-methyl-N-nitrosourea (MNU) and N-ethyl-N-nitrosourea
(MNU) linear concentration response characteristics for tail moment values and
somatic mutations were observed when tobacco seedlings were treated under identical
conditions (Menke et al., 2000).