3. Results
3.1. Growth on liquid and solid phytate or phosphate-media
Results from the liquid-based screening at 48 h are presented in
Table 1.No isolate grewon Delft−liquidmediumwhereas all the tested
isolates grew on Delft+ medium (data not shown). P. kudriavzevii
isolates, except one strain (M16), grew better than any other species
in liquidmedium, reaching an actualOD600 between 1.99 and 2.56 within
48 h and an OD600 ratio Delft Phy/Delft+ between 1.16 and 0.90
(Table 1). Results from the solid-medium confirmed the observations
from the liquid-medium, except for some strains of S. cerevisiae and Cl.
lusitaniae, which did not grow on plates as expected from the results
of the liquid assay (Supplementary material, Table S1). Fig. 1 presents
the results performed in liquid media for the five strains selected for
the PHYPk expression study i.e. G6, G5, M31, M30 and M16. As seen in
Fig. 1, although the OD600 of the four positive strains at t48 was almost
equal, their growth curves in Delft Phy and Delft+ differed. Strains G6
and G5 started growing in Delft Phy and Delft+ at t12 while M31 at t8
andM30 at t10. The growth curve ofM30 in Delft Phy liquid mediumdiffered
fromG6,G5 andM31. The strain chosen as negative control (M16)
was not able to growin the Delft Phymediumwhile it starts growing in
Delft+ at t14 (Fig. 1).