contained 250 nulliparous female mosquitoes (5 to 7 day-old). If at least two mosquitoes landed on or bit the arm, the repellency test was then continued. The test continued unit as least two bites occurred in a three-minute period. If no
mosquitoes bit or landed during the three-minute period, the arm was withdrawn from the cage. The repellency
test period was carried out every 30 min until fewer than 2 mosquitoes bit or land during the three-minute study
period at which time the repellency test was stopped. The time between application of the repellents was recorded as
the protection time