Samples of the diluted culture were subjected to either low voltage (18 V) or conventional heating (300°C) over a hotplate stirrer. Temperature was monitored on test and control samples to achieve an endpoint of 90°C. Samples were taken at regular intervals, plated onto MRS agar and incubated for 72 hours at 35°C to compare plate count expressed as colony forming units per milliliter (cfu/mL). Temperature was uniform throughout the ohmically heated sample and reached the endpoint more quickly than the conventionally heated sample, which also had cold spots. The total plate count at the end of the experiment was less for the ohmically heated sample versus the conventionally heated sample. Ohmic heating was more effective in inactivation of Lactobacillus acidophilus than conventional heating, most likely due to the more rapid and uniform heating of the sample, and possible electroporation of the cells.