2.3. Spectroscopy AOAC method
According to AOAC method (AOAC, 1958, 1973) one gram of
well mixed egg yolk from each egg was taken in a conical flask
where acetone was added in two steps, first 1 ml to make a smooth
past and thereafter 50 ml. The solution was well mixed and filtered
(equivalent to Whatman Nr. 4). After washing the filter with acetone,
the recovered acetone was diluted to 100 ml. Yolk pigmentation
equivalent to lg ß-carotene/g sample was measured on a
spectrophotometer at 450 nm wavelength