Nodal segments were cultured on ¾ Murashige and Skoog (Murashige and Skoog, 1962) media supplement for the initiation of Phaius tankervilliae with 3% sucrose and 0.8% agar. After adding the hormones, the pH of medium was adjusted to 5.75. The culture containing media were autoclaved at 121°C for 20 min. All the cultures were incubated in a culture chamber maintained at a temperature of 23-25°C under 16-h photoperiod provided by cool white fluorescent tubes.