In the present study, pH-stat methodwas calibrated by TNBS and OPAreactions for control of hydrolysis of corn gluten
by Alcalase. Hydrolysis experiments were conducted in a batch reactor at the temperature of 50 ◦C for various pH
values, and at pH 7 for 40 and 60 ◦C. The calibration was characterized by good linear correlations for both methods.