The PCR cycling scheme was as follows: 95 ◦C for
3.5 min, followed by 34 cycles at 94 ◦C (30 s), 55 ◦C (1min), 72 ◦C
(1.5 min) and a final extension step at 72 ◦C for 10 min. Each sample
was amplified in a separate reaction mixture containing uniquely
barcoded right primer, containing the MID sequence used in next
steps of analysis to assign sequences to particular samples.