Ten ml of blood was taken for each sample and transferred into an ice-chilled tube containing K3-EDTA. The tubes were centrifuged at 1500 g at 4 oC for 20 min and stored at-80 °C until assayed.
Ten ml of blood was taken for each sample and transferred into an ice-chilled tube containing K3-EDTA. The tubes were centrifuged at 1500 g at 4 oC for 20 min and stored at-80 °C until assayed.