Actinomycete isolates were evaluated for their antifungal
activity against FOC (Race 1; causal agent of Fusarium wilt in
chickpea), Rhizoctonia bataticola (Taub) Butter (three strains viz.
RB-6, RB-24 and RB-115; causal agent of dry root rot in chickpea)
and Macrophomina phaseolina (Tassi) Goid. (causal agent of charcoal
rot in sorghum) by dual-culture assay. FOC and all the three
strains of R. bataticola were acquired from the Legumes Pathology
Division, ICRISAT, Patancheru, India and M. phaseolinawas acquired
from the Directorate of Sorghum Research, Hyderabad, India.
A fungal disk (FOC/R. bataticola) of 6 mm dia. was placed on one
edge (1 cm from the corner) of the glucose casamino acid yeast
extract (GCY agar; Anjaiah et al., 1998) plate, and actinomycete
isolate was streaked on the other edge of the plate (1 cm from the
corner), followed by incubation at 28 2 C for four days or until
the pathogen covered the entire plate in the control plate. Inhibition
of fungal mycelium (halo zone) around the actinomycete
colony was noted as positive and the inhibition zone measured.
Actinomycete isolates were evaluated for their antifungalactivity against FOC (Race 1; causal agent of Fusarium wilt inchickpea), Rhizoctonia bataticola (Taub) Butter (three strains viz.RB-6, RB-24 and RB-115; causal agent of dry root rot in chickpea)and Macrophomina phaseolina (Tassi) Goid. (causal agent of charcoalrot in sorghum) by dual-culture assay. FOC and all the threestrains of R. bataticola were acquired from the Legumes PathologyDivision, ICRISAT, Patancheru, India and M. phaseolinawas acquiredfrom the Directorate of Sorghum Research, Hyderabad, India.A fungal disk (FOC/R. bataticola) of 6 mm dia. was placed on oneedge (1 cm from the corner) of the glucose casamino acid yeastextract (GCY agar; Anjaiah et al., 1998) plate, and actinomyceteisolate was streaked on the other edge of the plate (1 cm from thecorner), followed by incubation at 28 2 C for four days or untilthe pathogen covered the entire plate in the control plate. Inhibitionof fungal mycelium (halo zone) around the actinomycetecolony was noted as positive and the inhibition zone measured.
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